Synaptotagmin-8 Polyclonal antibody, PBS Only

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  WB analysis using 20671-1-AP (same clone as 20671-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20671-1-AP (Synaptotagmin-8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20671-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 20671-1-AP (same clone as 20671-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Synaptotagmin-8 antibody (20671-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20671-1-PBS in a different storage buffer formulation.

Synaptotagmin-8 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 20671-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

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Product Information

20671-1-PBS targets Synaptotagmin-8 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: synaptotagmin VIII
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 40 kDa, 50-54 kDa
• GenBank Accession Number: NM_138567
• Gene Symbol: SYT8
• Gene ID (NCBI): 90019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NBV8
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT8 (synaptotagmin-8) is related to insulin secretion, and its expression is positively regulated by the insulin promoter (PMID: 21336277). Due to alternative splicing, SYT8 may produce proteins with molecular weights between 25 and 40 to 44 kDa. An additional band of 50 kDa could also be detected (PMID: 16386321)