Synaptotagmin-5 Recombinant antibody

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  WB analysis using 84452-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 84452-1-RR

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84452-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 84452-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84452-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 84452-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 84452-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 84452-1-RR

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptotagmin-5 antibody (84452-1-RR, Clone: 241452E3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84452-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84452-1-RR against Human Synaptotagmin-5 were performed. The affinity constant is 0.131 nM.

Synaptotagmin-5 {ptg:Brand} Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 84452-1-RR

Clone No.241452E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

SytV, SYT5, Synaptotagmin V, 241452E3

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue, human kidney tissue, HUVEC cells, SW 1990 cells, BxPC-3 cells
• Positive IHC detected in: mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84452-1-RR targets Synaptotagmin-5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Synaptotagmin-5 fusion protein Ag12677
• Full Name: synaptotagmin V
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC046157
• Gene Symbol: Synaptotagmin-5
• Gene ID (NCBI): 6861
• RRID: AB_3671978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O00445
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-5 (SYT5) belongs to the synaptotagmin family. Synaptotagmin-5 can function as an exocytosis regulator (PMID: 27793683) and is a protein potentially associated with the pathology of schizophrenia (PMID: 32860155).