Synaptotagmin-10 Polyclonal antibody

Synaptotagmin-10 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20881-1-AP

Synonyms

SytX, SYT10, Synaptotagmin X

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Validation Data Gallery

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  WB analysis using 20881-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20881-1-AP (SYT10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20881-1-AP targets Synaptotagmin-10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Synaptotagmin-10 fusion protein Ag15018
• Full Name: synaptotagmin X
• Calculated Molecular Weight: 523 aa, 59 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC101024
• Gene Symbol: Synaptotagmin-10
• Gene ID (NCBI): 341359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT10 (synaptotagmin-10) Syt10 functions as a Ca2+ sensor to trigger IGF-1 exocytosis in neurons. Loss of Syt10 impairs activity-dependent IGF-1 secretion in olfactory bulb neurons and results in smaller neurons and an overall reduction in the number of synapses (PMID: 21496647).