Synaptophysin Recombinant antibody, PBS Only (Capture/Detector)

Synaptophysin Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240162C3

Cat no : 82900-4-PBS

Synonyms

Synaptophysin; SYP, SYP, SYN, MRX96, 240162C3

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00345-4

  Sandwich ELISA standard curve of MP00345-4, Human Synaptophysin Recombinant Matched Antibody Pair - PBS only. 82900-8-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag11781. 82900-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

• 

  Sample test of MP00345-4

  The mean Synaptophysin concentration was determined to be 518.35 ng/mL in SH-SY5Y cell extract based on a 2.2 mg/mL extract load.

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  Sample test of MP00345-4

  Serum of sixteen individual healthy human donors was measured. The Synaptophysin concentration of detected samples was determined to be 0.63 ng/mL with a range of 0.40 - 1.04 ng/mL.

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  Cytometric bead array standard curve of MP00345-1

  Cytometric bead array standard curve of MP00345-1, Synaptophysin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82900-4-PBS. Detection antibody: 82900-2-PBS. Standard: Ag11781. Range: 0.625-80 ng/mL.

• 

  Cytometric bead array standard curve of MP00345-2

  Cytometric bead array standard curve of MP00345-2, Synaptophysin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82900-5-PBS. Detection antibody: 82900-4-PBS. Standard: Ag11781. Range: 0.625-80 ng/mL.

• 

  Cytometric bead array standard curve of MP00345-3

  Cytometric bead array standard curve of MP00345-3, Synaptophysin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82900-3-PBS. Detection antibody: 82900-4-PBS. Standard: Ag11781. Range: 0.625-80 ng/mL.

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Product Information

82900-4-PBS targets Synaptophysin as part of a matched antibody pair:

MP00345-1: 82900-4-PBS capture and 82900-2-PBS detection (validated in Cytometric bead array)

MP00345-2: 82900-5-PBS capture and 82900-4-PBS detection (validated in Cytometric bead array)

MP00345-3: 82900-3-PBS capture and 82900-4-PBS detection (validated in Cytometric bead array)

MP00345-4: 82900-8-PBS capture and 82900-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Synaptophysin fusion protein Ag11781
• Full Name: synaptophysin
• Calculated Molecular Weight: 313 aa, 34 kDa
• GenBank Accession Number: BC064550
• Gene Symbol: Synaptophysin
• Gene ID (NCBI): 6855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.