Synaptophysin Monoclonal antibody, PBS Only

Synaptophysin Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat, Rabbit

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4E12C4

Cat no : 67864-1-PBS

Synonyms

synaptophysin, SYP

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Validation Data Gallery

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  WB analysis using 67864-1-Ig (same clone as 67864-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67864-1-Ig (Synaptophysin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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  WB analysis using 67864-1-Ig (same clone as 67864-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67864-1-Ig (Synaptophysin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67864-1-Ig (same clone as 67864-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67864-1-Ig (same clone as 67864-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse pancreas using 67864-1-Ig (same clone as 67864-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 67864-1-Ig (same clone as 67864-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67864-1-Ig (same clone as 67864-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 67864-1-Ig (same clone as 67864-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 67864-1-Ig (same clone as 67864-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67864-1-PBS targets Synaptophysin in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Pig, Mouse, Rat, Rabbit samples.

• Tested Reactivity: Human, Pig, Mouse, Rat, Rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Synaptophysin fusion protein Ag11803
• Full Name: synaptophysin
• Calculated Molecular Weight: 313 aa, 34 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC064550
• Gene Symbol: SYP
• Gene ID (NCBI): 6855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)