Stomatin Monoclonal antibody

Stomatin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A4D6

Cat no : 67894-1-Ig

Synonyms

BND7, EPB7, EPB72, Protein 7.2b, STOM, stomatin

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Validation Data Gallery

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  WB analysis of human placenta using 67894-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67894-1-Ig (Stomatin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 67894-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67894-1-Ig (Stomatin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67894-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67894-1-Ig (Stomatin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood platelets using 67894-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67894-1-Ig (Stomatin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 67894-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67894-1-Ig (Stomatin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue, HeLa cells, human peripheral blood platelets, human testis tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67894-1-Ig targets Stomatin in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Stomatin fusion protein Ag2676
• Full Name: stomatin
• Calculated Molecular Weight: 288 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC010703
• Gene Symbol: Stomatin
• Gene ID (NCBI): 2040
• RRID: AB_2918650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)