Spartin/SPG20 Recombinant antibody

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  WB analysis using 85938-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85938-2-RR (Spartin/SPG20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85938-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85938-2-RR against Human Spartin/SPG20 were performed. The affinity constant is 1.66 nM.

Spartin/SPG20 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85938-2-RR

Clone No.250440A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SPG20, KIAA0610, SPART, Spartin, SPG 20

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, PANC-1 cells, HL-60 cells, MDA-MB-231 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85938-2-RR targets Spartin/SPG20 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Spartin/SPG20 fusion protein Ag4815
• Full Name: spastic paraplegia 20 (Troyer syndrome)
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 75-84 kDa
• GenBank Accession Number: BC047083
• Gene Symbol: Spartin
• Gene ID (NCBI): 23111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N0X7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPG20 (spastic paraplegia 20) gene encodes a multifunctional Spartin protein. SPG20 protein is highly expressed in adipose tissue and may be implicated in endosomal trafficking and microtubule dynamics. SPG20 is mutated in Troyer syndrome, a hereditary spastic paraplegia defined by degeneration of upper motor neurons, recent study showed that regulation of SPG20 on mitochondrial calcium homeostasis may contribute to the pathophysiology of Troyer syndrome. Hypermethylation of SPG20 promoter, found in colorectal cancer patients, may play a role in cytokinesis arrest in colorectal tumorigenesis.