Spag8 Polyclonal antibody

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  WB analysis using 30287-1-AP

  rat testis tissue lysates were subjected to SDS PAGE followed by western blot with 30287-1-AP (Spag8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30287-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 30287-1-AP (Spag8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 30287-1-AP

  IP result of anti-Spag8 (IP:30287-1-AP, 4ug; Detection:30287-1-AP 1:3000) with mouse testis tissue lysate 1120 ug.

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  IHC staining of mouse testis using 30287-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30287-1-AP (Spag8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 30287-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30287-1-AP (Spag8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Spag8 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 30287-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse

Applications

WB, IP, IHC, ELISA

Synonyms

MH SPAG8, Spag8, sperm associated antigen 8

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30287-1-AP targets Spag8 in WB, IP, IHC, ELISA applications and shows reactivity with rat, mouse samples.

• Tested Reactivity: rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Spag8 fusion protein Ag31603
• Full Name: sperm associated antigen 8
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC150868
• Gene Symbol: Spag8
• Gene ID (NCBI): 433700
• RRID: AB_3086286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q3V0Q6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.