Sortilin Monoclonal antibody

Sortilin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2B11B9

Cat no : 68007-1-Ig

Synonyms

100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3, SORT1, Sortilin, sortilin 1



Tested Applications

Positive WB detected inROS1728 cells, SK-N-SH cells, SH-SY5Y cells, pig brain tissue, rabbit brain, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inmouse brain tissue, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68007-1-Ig targets Sortilin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Sortilin fusion protein Ag28043
Full Name sortilin 1
Calculated Molecular Weight 831 aa, 92 kDa
Observed Molecular Weight95-100 kDa
GenBank Accession NumberBC023542
Gene Symbol Sortilin
Gene ID (NCBI) 6272
RRIDAB_2918754
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.

Protocols

Product Specific Protocols
WB protocol for Sortilin antibody 68007-1-IgDownload protocol
IHC protocol for Sortilin antibody 68007-1-IgDownload protocol
IF protocol for Sortilin antibody 68007-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols