SignalBright Max Chemiluminescent Substrate

Ultra-Sensitivity:

Mid to low femtogram

Signal duration:

More than 12 hours

Compatibility:

suitable for PVDF and nitrocellulose membranes

Publications

3

Cat no : PK10013

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Validation Data Gallery

• 

  Serial dilutions of RUVBL primary antibody (10210-2-AP)
  Primary: Proteintech RUVBL1 (10210-2-AP) at various dilutions (see image)
  Secondary: Proteintech HRP-conjugated Goat Anti-Rabbit IgG (SA00001-2); 1:6,000
  Exposure time: 1 min
  Chemiluminescent substrates from leading competitor, SignalBright Pro (PK10011), SignalBright Max (PK10013)

• 

  Serial dilutions of HEK-293 cell lysates
  Primary: Proteintech RUVBL1 (10210-2-AP); 1:8,000
  Secondary: Proteintech HRP-conjugated Goat Anti-Rabbit IgG (SA00001-2); 1:6,000
  Exposure time: 1 min
  Chemiluminescent substrates from leading competitor, SignalBright Pro (PK10011), SignalBright Max (PK10013)

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Product Information

SignalBright Max is a luminol-based, ultra-sensitive chemiluminescent substrate for HRP (horseradish peroxidase) which provides a much higher sensitivity over traditional ECL reagents. SignalBright Max provides increased sensitivity over SignalBright Pro, with mid to low femtogram detection levels in Western Blot and ELISA analysis.

Key Characteristics:

· Ultra-Sensitivity: Mid to low femtogram

· Signal duration: > 12 hours

· Compatibility: suitable for PVDF and nitrocellulose membranes

· Comparable sensitivity to SuperSignal™ West Femto

Instructions for Use

SignalBright Max Chemiluminescent Detection Kit is prepared by combining a 1:1 ratio of Chemiluminescent Substrate Solution and  Stable Peroxide Solution to create a working solution. Allow the working solution to come to room temperature before applying to the membrane, make sure this is done in darkness. Apply a minimum of 0.1ml of the working solution per membrane cm². Leave 5 minutes for signal to develop prior to imaging. Signal can be imaged using an X-ray film or a Chemiluminescent Imager.

· Recommended primary antibody concentration: 10-200 ng/ml

· Recommended HRP-conjugated secondary antibody concentration: 1-10 ng/ml

Product Information

Cat.	Size	Content
PK10013	20mL	10mL Chemiluminescent Substrate Solution
		10mL Stable Peroxide Solution
	100mL	50mL Chemiluminescent Substrate Solution
		50mL Stable Peroxide Solution

Storage

Store at 4ºC. Expires 1 year after purchase.

Protocol

SignalBright Max Chemiluminescent Substrate Protocol

Cited in Article as

PK10013, SignalBright Max Chemiluminescent Substrate, Proteintech, IL, USA

Publications

Application	Title
WB	Proc Natl Acad Sci U S A; The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation; Authors - Magda Wąchalska
WB	bioRxiv; The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation; Authors - Magda Wąchalska
	Chem Biol Interact; Berberine hydrochloride ameliorates PM2.5-induced pulmonary fibrosis in mice through inhibiting oxidative stress and inflammatory; Authors - Jiali Zhao