Filter: AllWBIHC

WB analysis using 66542-1-Ig (same clone as 66542-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66542-1-Ig (Septin 7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 66542-1-Ig (same clone as 66542-1-PBS)
WB result of Septin 7 antibody (66542-1-Ig; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 7 transfected HepG2 cells. This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66542-1-Ig (same clone as 66542-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66542-1-Ig (same clone as 66542-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 66542-1-Ig (same clone as 66542-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66542-1-Ig (same clone as 66542-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66542-1-Ig (same clone as 66542-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66542-1-Ig (same clone as 66542-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 66542-1-Ig (same clone as 66542-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Septin 7 Monoclonal antibody, PBS Only

Septin 7 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66542-1-PBS

Clone No.1G12D1

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

septin7, SEPT7, SEPT 7, CDC3, CDC10 protein homolog

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66542-1-PBS targets Septin 7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Septin 7 fusion protein Ag4309 Predict reactive species
Full Name	septin 7
Calculated Molecular Weight	51 kDa
Observed Molecular Weight	48-51 kDa
GenBank Accession Number	BC025987
Gene Symbol	Septin 7
Gene ID (NCBI)	989
RRID	AB_2881904
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q16181
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Septins (SEPTs) are members of a conserved family of cytoskeletal GTPases involved in various cellular processes, including cytoskeleton organization, cytokinesis, and membrane dynamics. Septin 7 is a unique septin required for filament formation. It has also been reported to be involved in migration and invasion in various cancer cells, including breast cancer and glioma.