Septin 5 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Septin 5 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11631-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of Jurkat using 11631-1-AP (same clone as 11631-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11631-1-AP (same clone as 11631-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 11631-1-AP (same clone as 11631-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 11631-1-AP (same clone as 11631-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 11631-1-AP (same clone as 11631-1-PBS)

  IP result of anti-Septin 5 (IP:11631-1-AP, 3ug; Detection:11631-1-AP 1:300) with mouse brain tissue lysate 1000ug. This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11631-1-AP (same clone as 11631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11631-1-AP (same clone as 11631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

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Product Information

11631-1-PBS targets Septin 5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Septin 5 fusion protein Ag2205
• Full Name: septin 5
• Calculated Molecular Weight: 369 aa, 43 kDa
• Observed Molecular Weight: 40-43 kDa
• GenBank Accession Number: BC025261
• Gene Symbol: Septin 5
• Gene ID (NCBI): 5413
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Septin 5, a septin family member formerly called cell division control related protein 1 (CDCrel), is encoded in the 200 kb region and is abundantly expressed in the developing and adult brains of mice and humans. Although its constitutive deletion does not cause detectible structural or developmental abnormalities in the mouse brain, Septin 5 has been implicated in subtle neuronal and behavioral functions. Septin 5 is expressed in presynaptic terminals and exerts negative regulatory control over neurotransmitter release by binding to SNARE-protein, which has been implicated in neuropsychiatric disorders (PMID: 19240081). Septin 5 has 2 isoforms with the molecular mass of 39 and 43 kDa.