RISC Monoclonal antibody

RISC Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1C6A7

Cat no : 60086-1-Ig

Synonyms

2410018F01Rik, 4833411K15Rik, AI957256, Risc, Scpep1, serine carboxypeptidase 1

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Validation Data Gallery

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  WB analysis of human testis using 60086-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60086-1-Ig (RISC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 60086-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60086-1-Ig (RISC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 60086-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60086-1-Ig (RISC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 60086-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60086-1-Ig (RISC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 60086-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60086-1-Ig (RISC antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 60086-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60086-1-Ig (RISC antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of C6 using 60086-1-Ig

  Immunofluorescent analysis of C6 cells using 60086-1-Ig (RISC antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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Tested Applications

• Positive WB detected in: human testis tissue, human heart tissue, MCF-7 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

60086-1-Ig targets RISC in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: RISC fusion protein Ag0614
• Full Name: serine carboxypeptidase 1
• Calculated Molecular Weight: 452 aa, 51 kDa
• Observed Molecular Weight: 35-40 kDa/50-60 kDa
• GenBank Accession Number: AF330052
• Gene Symbol: Scpep1
• Gene ID (NCBI): 74617
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Scpep1(Retinoid-inducible serine carboxypeptidase) is a 51 kDa protein which is also named as RISC and belongs to the peptidase S10 family. This protein may be involved in vascular wall and kidney homeostasis.

Publications

Species	Application	Title
human	WB	Autophagy; Donor MSCs release apoptotic bodies to improve myocardial infarction via autophagy regulation in recipient cells.; Authors - Huan Liu