Sam50 Monoclonal antibody, PBS Only

Sam50 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Rat, Mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H6H9

Cat no : 67425-1-PBS

Synonyms

CGI 51, FLJ35825, FLJ42905, FLJ99036, OMP85, Sam50, SAMM50, TOB55, TRG 3, TRG3, YNL026W

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Validation Data Gallery

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  WB analysis using 67425-1-Ig (same clone as 67425-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67425-1-Ig (Sam50 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

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  WB analysis using 67425-1-Ig (same clone as 67425-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67425-1-Ig (Sam50 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67425-1-Ig (same clone as 67425-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67425-1-Ig (SAMM50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67425-1-Ig (same clone as 67425-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67425-1-Ig (SAMM50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67425-1-Ig (same clone as 67425-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67425-1-Ig (SAMM50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67425-1-Ig (same clone as 67425-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67425-1-Ig (same clone as 67425-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin was stained using CL594-phalloidin (red) and DNA was stained by DAPI (blue). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67425-1-PBS targets Sam50 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Pig, Rat, Mouse samples.

• Tested Reactivity: Human, Pig, Rat, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Sam50 fusion protein Ag15155
• Full Name: sorting and assembly machinery component 50 homolog (S. cerevisiae)
• Calculated Molecular Weight: 469 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC007830
• Gene Symbol: SAMM50
• Gene ID (NCBI): 25813
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).