Synaptotagmin-9 Monoclonal antibody

Synaptotagmin-9 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D2F2

Cat no : 68430-1-Ig

Synonyms

SytIX, SYT9, Synaptotagmin IX, 3D2F2

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Validation Data Gallery

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  WB analysis using 68430-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68430-1-Ig (Synaptotagmin-9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Tested Applications

• Positive WB detected in: rabbit retina tissue, rat retina tissue, mouse retina tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68430-1-Ig targets Synaptotagmin-9 in WB, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

• Tested Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Synaptotagmin-9 fusion protein Ag33194
• Full Name: synaptotagmin IX
• Calculated Molecular Weight: 491 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC029605
• Gene Symbol: Synaptotagmin-9
• Gene ID (NCBI): 143425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-9 (SYT9) is a tandem C2 domain Ca 2+ sensor for exocytosis in neuroendocrine cells (PMID: 36732068).