Synaptotagmin-14 Recombinant antibody, PBS Only

Synaptotagmin-14 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240866C2

Cat no : 83923-1-PBS

Synonyms

SytXIV, SYT14, Synaptotagmin XIV, 240866C2

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Validation Data Gallery

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  WB analysis using 83923-1-RR (same clone as 83923-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse cerebellum using 83923-1-RR (same clone as 83923-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 83923-1-RR (SYT14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 83923-1-RR (same clone as 83923-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 83923-1-RR (same clone as 83923-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83923-1-RR (same clone as 83923-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Synaptotagmin-14 Recombinant antibody (83923-1-RR, Clone:240866C2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

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Product Information

83923-1-PBS targets Synaptotagmin-14 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: synaptotagmin XIV
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: NM_153262
• Gene Symbol: Synaptotagmin-14
• Gene ID (NCBI): 255928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-14 (SYT14) is highly expressed in brain tissues. A homozygous mutation of SYT14 is involved in the pathogenesis of spinocerebellar ataxia (SCA) (PMID: 21835308).