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SYT1 Recombinant antibody, PBS Only

SYT1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240589F6

Cat no : 83637-4-PBS

Synonyms

Synaptotagmin 1, Synaptotagmin-1, synaptotagmin I, SVP65, P65

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Synaptotagmin-1 Recombinant antibody (83637-4-RR)

    WB analysis using 83637-4-RR (same clone as 83637-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83637-4-RR (Synaptotagmin-1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83637-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Synaptotagmin-1 Recombinant antibody (83637-4-RR)

    IF Staining of SH-SY5Y using 83637-4-RR (same clone as 83637-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Synaptotagmin-1 antibody (83637-4-RR, Clone: 240589F6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83637-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Synaptotagmin-1 Recombinant antibody (83637-4-RR)

    IF Staining of SH-SY5Y using 83637-4-RR (same clone as 83637-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Synaptotagmin-1 antibody (83637-4-RR, Clone: 240589F6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83637-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83637-4-RR

    Affinity and Kinetic Characterization of 83637-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83637-4-RR against Human Synaptotagmin-1 were performed. The affinity constant is 20.5 nM.

Product Information

The immunogen of 83637-4-PBS is SYT1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SYT1 fusion protein Ag5970
Full Name synaptotagmin I
Calculated Molecular Weight 48 kDa
GenBank Accession NumberBC058917
Gene Symbol Synaptotagmin-1
Gene ID (NCBI) 6857
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.