SYNPO2L Polyclonal antibody, PBS Only

SYNPO2L Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21480-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 21480-1-AP (same clone as 21480-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21480-1-AP (SYNPO2L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21480-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 21480-1-AP (same clone as 21480-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21480-1-AP (SYNPO2L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21480-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 21480-1-AP (same clone as 21480-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21480-1-AP (SYNPO2L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21480-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 21480-1-AP (same clone as 21480-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21480-1-AP (SYNPO2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21480-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 21480-1-AP (same clone as 21480-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21480-1-AP (SYNPO2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21480-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 21480-1-AP (same clone as 21480-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SYNPO2L antibody (21480-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21480-1-PBS in a different storage buffer formulation.

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Product Information

21480-1-PBS targets SYNPO2L in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SYNPO2L fusion protein Ag15683
• Full Name: synaptopodin 2-like
• Calculated Molecular Weight: 977 aa, 102 kDa
• Observed Molecular Weight: 79 kDa
• GenBank Accession Number: BC142635
• Gene Symbol: SYNPO2L
• Gene ID (NCBI): 79933
• RRID: AB_10860260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SYNPO2L belongs to the synaptopodin family and is an actin-associated protein that may play a role in modulating actin-based shape. SYNPO2L is involved in the positive regulation of Rho protein signal transduction and the positive regulation of stress fiber assembly. Loss-of-function variants in the SYNPO2L gene are associated with atrial fibrillation (PMID: 33768119).