SYNPO Recombinant antibody

SYNPO Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4K5

Cat no : 80721-1-RR

Synonyms

Synaptopodin, snaptopodin, KIAA1029, 4K5

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Validation Data Gallery

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  WB analysis using 80721-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80721-1-RR (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 80721-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 80721-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 80721-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 80721-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 80721-1-RR

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 80721-1-RR

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 80721-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 80721-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 80721-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 80721-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 80721-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80721-1-RR against Human SYNPO were performed. The affinity constant is 1.38 nM.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, pig brain tissue
• Positive IHC detected in: human kidney tissue, mouse brain tissue, mouse kidney tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:17800
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80721-1-RR targets SYNPO in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SYNPO fusion protein Ag31287
• Full Name: synaptopodin
• Calculated Molecular Weight: 929 aa, 99 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC146665
• Gene Symbol: SYNPO
• Gene ID (NCBI): 11346
• RRID: AB_2918907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)