SYNJ2 Polyclonal antibody

• 

  WB analysis using 30391-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30391-1-AP (SYNJ2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30391-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30391-1-AP (SYNJ2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse small intestine using 30391-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30391-1-AP (SYNJ2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 30391-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30391-1-AP (SYNJ2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-251 using 30391-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using SYNJ2 antibody (30391-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

SYNJ2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 30391-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

synaptojanin 2, Synaptojanin-2, Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 2, KIAA0348, INPP5H

---

Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells
• Positive IHC detected in: mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30391-1-AP targets SYNJ2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SYNJ2 fusion protein Ag33002
• Full Name: synaptojanin 2
• Calculated Molecular Weight: 1496 aa, 166 kDa
• Observed Molecular Weight: 143 kDa, 160 kDa
• GenBank Accession Number: BC043277
• Gene Symbol: SYNJ2
• Gene ID (NCBI): 8871
• RRID: AB_3086305
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15056
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYNJ2(Synaptojanin-2) is also named as KIAA0348 and belongs to the synaptojanin family. SYNJ2 encodes an inositol polyphosphate phosphatase that functions in recycling neurotransmitter vesicles and is implicated in spermatogenesis(PMID: 24103750). This protein has 3 isoforms produced by alternative splicing.