Tested Applications
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IF/ICC detected in | U-251 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
85045-1-RR targets SYNGAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Peptide Predict reactive species |
Full Name | synaptic Ras GTPase activating protein 1 homolog (rat) |
Calculated Molecular Weight | 1343 aa, 148 kDa |
Observed Molecular Weight | 140 kDa |
GenBank Accession Number | NM_006772 |
Gene Symbol | SYNGAP1 |
Gene ID (NCBI) | 8831 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q96PV0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SYNGAP1, also named as KIAA1938, is the major constituent of the PSD essential for postsynaptic signaling. It's an inhibitory regulator of the Ras-cAMP pathway. SYNGAP1 is a member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. SYNGAP1 regulates AMPAR-mediated miniature excitatory postsynaptic currents. SYNGAP1 may be involved in certain forms of brain injury, leading to long-term learning and memory deficits Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5).
Protocols
Product Specific Protocols | |
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WB protocol for SYNGAP1 antibody 85045-1-RR | Download protocol |
IF protocol for SYNGAP1 antibody 85045-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |