SYNGAP1 Polyclonal antibody

SYNGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 19739-1-AP

Synonyms

Synaptic Ras-GAP 1, Synaptic Ras GTPase-activating protein 1, RASA 1, Ras/Rap GTPase-activating protein SynGAP, Ras GTPase activating protein SynGAP

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Validation Data Gallery

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  WB analysis using 19739-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19739-1-AP (SYNGAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19739-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19739-1-AP (SYNGAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 19739-1-AP

  IP result of anti-SYNGAP1 (IP:19739-1-AP, 4ug; Detection:19739-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 19739-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19739-1-AP (SYNGAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19739-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19739-1-AP (SYNGAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 19739-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using 19739-1-AP (SYNGAP1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissues, pig brain tissues
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below

Product Information

19739-1-AP targets SYNGAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: synaptic Ras GTPase activating protein 1 homolog (rat)
• Calculated Molecular Weight: 1343 aa, 148 kDa
• Observed Molecular Weight: 148 kDa
• GenBank Accession Number: NM_006772
• Gene Symbol: SYNGAP1
• Gene ID (NCBI): 8831
• RRID: AB_10638472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYNGAP1, also named as KIAA1938, is the major constituent of the PSD essential for postsynaptic signaling. It's an inhibitory regulator of the Ras-cAMP pathway. SYNGAP1 is a member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. SYNGAP1 regulates AMPAR-mediated miniature excitatory postsynaptic currents. SYNGAP1 may be involved in certain forms of brain injury, leading to long-term learning and memory deficits Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5).

Publications

Species	Application	Title
mouse	WB	Front Aging Neurosci; Impaired Learning and Memory Ability Induced by a Bilaterally Hippocampal Injection of Streptozotocin in Mice: Involved With the Adaptive Changes of Synaptic Plasticity.; Authors - Cong-Cong Qi
mouse	WB	Neurochem Int; Resveratrol ameliorates learning and memory impairments induced by bilateral hippocampal injection of streptozotocin in mice.; Authors - Ya-Dong Wei
mouse	WB,IHC	Eur J Neurosci; SynGAP is expressed in the murine suprachiasmatic nucleus and regulates circadian-gated locomotor activity and light-entrainment capacity.; Authors - Sydney Aten
mouse	WB	Neuroscience; Further Studies on the Role of BTBD9 in the Cerebellum, Sleep-like Behaviors and the Restless Legs Syndrome; Authors - Shangru Lyu
mouse	WB	Mol Neurobiol; SNX17 Mediates Dendritic Spine Maturation via p140Cap; Authors - Qiuyan Cui
mouse	WB	J Nutr Biochem; Hippocampal proteomic changes in high-fat diet-induced obese mice associated with memory decline; Authors - Ping Lu