SYN1-Specific Polyclonal antibody

SYN1-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

50

Reviews (2)

Cat no : 20258-1-AP

Synonyms

Brain protein 4.1, SYN1, SYN1a, SYN1b, SYN1-Specific, Synapsin 1, synapsin I, SYNI

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Validation Data Gallery

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  WB analysis of mouse brain using 20258-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20258-1-AP (SYN1-Specific Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 20258-1-AP

  IP result of anti-SYN1-Specific (IP:20258-1-AP, 4ug; Detection:20258-1-AP 1:3000) with rat brain tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 20258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20258-1-AP (SYN1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20258-1-AP (SYN1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 40 publications below
• IHC: See 2 publications below
• IF: See 17 publications below

Product Information

20258-1-AP targets SYN1-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: synapsin I
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: NM_006950
• Gene Symbol: SYN1
• Gene ID (NCBI): 6853
• RRID: AB_2800493
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYN1, also named as Brain protein 4.1, belongs to the synapsin family. SYN1 is a neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders (XELBD).

Publications

Species	Application	Title
mouse	WB	Environ Health Perspect; Evaluation of Neurotoxicity in BALB/c Mice following Chronic Exposure to Polystyrene Microplastics; Authors - Haibo Jin
rat	WB	Neurobiol Stress; Low-dose S-ketamine exerts antidepressant-like effects via enhanced hippocampal synaptic plasticity in postpartum depression rats.; Authors - Zhuoyu Ren
human	WB,IF	Prog Neurobiol; Islet Amyloid Polypeptide Triggers α-synuclein Pathology in Parkinson's Disease; Authors - Lanxia Meng
mouse	WB	Brain Behav Immun; Hippocampal alpha-synuclein mediates depressive-like behaviors.; Authors - Tingfu Du
mouse	WB	Sci Total Environ; Manganese-induced α-synuclein overexpression promotes the accumulation of dysfunctional synaptic vesicles and hippocampal synaptotoxicity by suppressing Rab26-dependent autophagy in presynaptic neurons; Authors - Zhuo Ma
mouse	WB	ACS Chem Neurosci; Merazin Hydrate Produces Rapid Antidepressant Effects Depending on Activating mTOR Signaling by Upregulating Downstream Synaptic Proteins in the Hippocampus.; Authors - Chao Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Azita (Verified Customer) (05-31-2021)

Human primary cortical cells went trough ICC and the SYN1 antibody at the 1/50 dilution and MAP2 antibody at the 1/1000 dilution were used overnight at 4°C.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/50
• Cell Tissue Type: Human primary cortical cells

SYN1-Specific Antibody Immunohistochemistry validation (1/50 dilution) in Human primary cortical cells (Cat no:20258-1-AP)

FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain