SYK Polyclonal antibody

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• KD/KO Validated

SYK Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14858-1-AP

Synonyms

Tyrosine-protein kinase SYK, Tyrosine protein kinase SYK, spleen tyrosine kinase, p72-Syk, IMD82

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Validation Data Gallery

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  WB analysis of Raji using 14858-1-AP

  WB result of SYK antibody (14858-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SYK transfected Raji cells.

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  WB analysis using 14858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14858-1-AP (SYK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14858-1-AP (SYK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14858-1-AP (SYK antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14858-1-AP (SYK antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14858-1-AP (SYK antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14858-1-AP (SYK antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human appendicitis using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 14858-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14858-1-AP (SYK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Ramos using 14858-1-AP

  1x10^6 Ramos cells were intracellularly stained with 0.4 ug SYK Polyclonal antibody (14858-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, Ramos cells
• Positive IHC detected in: human liver cancer tissue, human appendicitis tissue, human spleen tissue, human kidney tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

14858-1-AP targets SYK in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SYK fusion protein Ag6657
• Full Name: spleen tyrosine kinase
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 66-72 kDa
• GenBank Accession Number: BC001645
• Gene Symbol: SYK
• Gene ID (NCBI): 6850
• RRID: AB_2878086
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYK belongs to the protein kinase superfamily, Tyr protein kinase family and SYK/ZAP-70 subfamily. It is positive effector of BCR-stimulated responses. SYK couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway It phosphorylates USP25 and regulates its intracellular levels. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660) SYK can be detected 66-72 kDa in human and mouse (PMID: 37661351).

Publications

Species	Application	Title
mouse	WB	Food Funct; Synergistic effects of L-theanine and epigallocatechin gallate in alleviating ovalbumin allergy by regulating intestinal immunity through inhibition of mast cell degranulation; Authors - Wei Xu
mouse	WB	Int Immunopharmacol; SAP130 released by ferroptosis tubular epithelial cells promotes macrophage polarization via Mincle signaling in sepsis acute kidney injury; Authors - Jing Zhang
mouse	IHC	J Ethnopharmacol; Yiqi Kaimi prescription regulates protein phosphorylation to promote intestinal motility in slow transit constipation; Authors - Yi-Bo Yao
mouse	WB	Brain Res; Inhibition of TREM-1 alleviates neuroinflammation by modulating microglial polarization via SYK/p38MAPK signaling pathway after traumatic brain injury; Authors - Tianqi Zhao
human	IHC	Front Genet; SYK Is Associated With Malignant Phenotype and Immune Checkpoints in Diffuse Glioma.; Authors - Quanwei Zhou
mouse	WB	Animals (Basel); Equus β-Defensin-1 Regulates Innate IMMUNE Response in S. aureus-Infected Mouse Monocyte Macrophage; Authors - Le Pei