SYAP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SYAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16272-1-AP

Synonyms

PRO3113, SYAP1, Synapse associated protein 1

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Validation Data Gallery

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  WB analysis using 16272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16272-1-AP (SYAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16272-1-AP (SYAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 16272-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16272-1-AP (SYAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 16272-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16272-1-AP (SYAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 16272-1-AP

  IP result of anti-SYAP1 (IP:16272-1-AP, 4ug; Detection:16272-1-AP 1:500) with L02 cells lysate 1800ug.

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  IHC staining of human cervical cancer using 16272-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16272-1-AP (SYAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 16272-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16272-1-AP (SYAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 16272-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16272-1-AP (SYAP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 16272-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16272-1-AP (SYAP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 16272-1-AP

  Immunofluorescent analysis of HepG2 cells using 16272-1-AP (SYAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 16272-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SYAP1 antibody (16272-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 16272-1-AP

  Immunofluorescent analysis of A431 cells using 16272-1-AP (SYAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, SMMC-7721 cells, A431 cells, mouse lung tissue, HepG2 cells, L02 cells, mouse liver tissue, rat lung tissue
• Positive IHC detected in: human cervical cancer tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Product Information

16272-1-AP targets SYAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SYAP1 fusion protein Ag9342
• Full Name: synapse associated protein 1, SAP47 homolog (Drosophila)
• Calculated Molecular Weight: 352 aa, 40 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC014657
• Gene Symbol: SYAP1
• Gene ID (NCBI): 94056
• RRID: AB_2878236
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYAP1(Synapse-associated protein 1) is a human homologue of the Drosophila SAP47 (synapse associated protein), which is recognized by a monoclonal antibody that selectively stain synaptic terminals.This protein is a 352 amino acid protein that is ubiquitously expressed in adult tissues. SYAP1 contains one BSD domain which is is an approximately 60 amino acid long protein domain named after the BTF2-like transcription factors, Synapse-associated proteins and DOS2-like proteins in which it is found.

Publications

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
mouse	WB,IF	Histochem Cell Biol; Initial characterization of a Syap1 knock-out mouse and distribution of Syap1 in mouse brain and cultured motoneurons.; Authors - Dominique Schmitt ; KO Validated
mouse	IF	Biol Open; An essential role of the mouse synapse-associated protein Syap1 in circuits for spontaneous motor activity and rotarod balance.; Authors - Cora R von Collenberg