SV2B Polyclonal antibody
SV2B Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 14624-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
14624-1-AP targets SV2B in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SV2B fusion protein Ag6137 |
Full Name | synaptic vesicle glycoprotein 2B |
Calculated Molecular Weight | 77 kDa |
Observed Molecular Weight | 77 kDa |
GenBank Accession Number | BC030011 |
Gene Symbol | SV2B |
Gene ID (NCBI) | 9899 |
RRID | AB_10641040 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for SV2B antibody 14624-1-AP | Download protocol |
IHC protocol for SV2B antibody 14624-1-AP | Download protocol |
IP protocol for SV2B antibody 14624-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Neurol Environmental Enrichment Upregulates Striatal Synaptic Vesicle-Associated Proteins and Improves Motor Function. | ||
Neurosci Bull Mutant Huntingtin Causes a Selective Decrease in the Expression of Synaptic Vesicle Protein 2C. | ||