Various lysates were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 12826-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12826-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 12826-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
PC-3 cells were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa cells using 12826-1-AP
HeLa cell were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:500.
IP result of anti-SUPV3L1 (IP:12826-1-AP, 4ug; Detection:12826-1-AP 1:300) with HeLa cells lysate 1200ug.
IHC staining of human stomach cancer using 12826-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12826-1-AP (SUPV3L1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12826-1-AP (SUPV3L1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 12826-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12826-1-AP (SUPV3L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12826-1-AP (SUPV3L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
SUPV3L1 is a major helicase player in mitochondrial RNA metabolism. It is involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PMID: 29967381).
Various lysates were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 12826-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12826-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 12826-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa cells using 12826-1-AP
HeLa cell were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:500.
IHC Figures
IHC staining of human stomach cancer using 12826-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12826-1-AP (SUPV3L1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HeLa using 12826-1-AP
IP result of anti-SUPV3L1 (IP:12826-1-AP, 4ug; Detection:12826-1-AP 1:300) with HeLa cells lysate 1200ug.
IP experiment of HeLa using 12826-1-AP
IP result of anti-SUPV3L1 (IP:12826-1-AP, 4ug; Detection:12826-1-AP 1:300) with HeLa cells lysate 1200ug.
IP experiment of HeLa using 12826-1-AP
IP result of anti-SUPV3L1 (IP:12826-1-AP, 4ug; Detection:12826-1-AP 1:300) with HeLa cells lysate 1200ug.
IF/ICC Figures
IF Staining of HeLa using 12826-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12826-1-AP (SUPV3L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
SUPV3L1 Polyclonal antibody
Catalog Number
12826-1-AP
Citations
2
Dilutions
WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:250-1:1000 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
