SUPT16H Polyclonal antibody

• Featured Product
• KD/KO Validated

SUPT16H Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28598-1-AP

Synonyms

FACTp140, FACT140, FACT 140 kDa subunit, Facilitates chromatin transcription complex subunit SPT16, Chromatin-specific transcription elongation factor 140 kDa subunit

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Validation Data Gallery

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  WB analysis of MCF-7 using 28598-1-AP

  WB result of SUPT16H antibody (28598-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUPT16H transfected MCF-7 cells.

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  WB analysis using 28598-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28598-1-AP (SUPT16H antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 28598-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28598-1-AP (SUPT16H antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 28598-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUPT16H antibody (28598-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, HeLa cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28598-1-AP targets SUPT16H in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUPT16H fusion protein Ag29459
• Full Name: suppressor of Ty 16 homolog (S. cerevisiae)
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: NM_007192
• Gene Symbol: SUPT16H
• Gene ID (NCBI): 11198
• RRID: AB_2918179
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUPT16H, also named as FACT140, FACTP140, SPT16 and CDC68, belongs to the peptidase M24 family and SPT16 subfamily. SUPT16H is a component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). It also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to SUPT16H.