SUMO2/3 Polyclonal antibody

• Featured Product
• KD/KO Validated

SUMO2/3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

16

Reviews (2)

Cat no : 11251-1-AP

Synonyms

SUMO2, SMT3B, Small ubiquitin-related modifier 2, Sentrin-2, Sentrin2

---

Validation Data Gallery

• 

  WB analysis using 11251-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11251-1-AP (SUMO2/3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11251-1-AP

  WB result of SUMO2/3 antibody (11251-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUMO2/3 transfected HEK-293 cells.

• 

  WB analysis using 11251-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11251-1-AP (SUMO2/3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse colon using 11251-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11251-1-AP (SUMO2/3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11251-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11251-1-AP (SUMO2/3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 11251-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SUMO2/3 antibody (11251-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HSC-T6 cells, HeLa cells, Jurkat cells, L02 cells, PC-12 cells
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Product Information

11251-1-AP targets SUMO2/3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUMO2/3 fusion protein Ag1778
• Full Name: SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 11-20 kDa
• GenBank Accession Number: BC016775
• Gene Symbol: SUMO2
• Gene ID (NCBI): 6613
• RRID: AB_2198405
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

Publications

Species	Application	Title
human	WB	Sci Adv; XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility; Authors - Ming Kuang
human	WB	Theranostics; Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation.; Authors - Shuai Huang
human	IP	mSystems; Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia.; Authors - Xiaoqing Liu
mouse	WB	iScience; Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress; Authors - Amr R Ghanam
mouse	IP	Int J Mol Med; SUMO2 modification of Aurora B and its impact on follicular development and atresia in the mouse ovary.; Authors - Jing Cao
mouse	WB,IP	Aging (Albany NY); SUMO1-regulated DBC1 promotes p53-dependent stress-induced apoptosis of lens epithelial cells; Authors - Yan Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Verdiana (Verified Customer) (03-02-2023)

Good bands. Little aspecific I think but the gels we use have a high resolution and also those were lympoblastoid cells and we know they can me strange. Anyway, it is a good antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Lymphoblastic cell line

SUMO2/3 Antibody Western Blot validation (1:1000 dilution) in Lymphoblastic cell line (Cat no:11251-1-AP)

FH

Shubham (Verified Customer) (03-13-2019)

Good

• Applications: Western Blot,
• Cell Tissue Type: AC16

SUMO2/3 Antibody Western Blot, validation ( dilution) in AC16 (Cat no:11251-1-AP)