SUMO2/3 Polyclonal antibody

• Featured Product
• KD/KO Validated

SUMO2/3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10947-1-AP

Synonyms

SUMO2, SMT3B, Small ubiquitin-related modifier 2, Sentrin-2, Sentrin2

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Validation Data Gallery

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  WB analysis of HEK-293 using 10947-1-AP

  WB result of SUMO2/3 antibody (10947-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUMO2/3 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 10947-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10947-1-AP (SUMO2/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10947-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10947-1-AP (SUMO2/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Recombinant protein using 10947-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 10947-1-AP (SUMO2/3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10947-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10947-1-AP (SUMO2/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10947-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10947-1-AP (SUMO2/3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10947-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10947-1-AP (SUMO2/3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10947-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUMO2/3 antibody (10947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, A549 cells, Recombinant protein protein
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

10947-1-AP targets SUMO2/3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUMO2/3 fusion protein Ag1388
• Full Name: SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC008450
• Gene Symbol: SUMO2
• Gene ID (NCBI): 6613
• RRID: AB_2198406
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

Publications

Species	Application	Title
human	WB,IF	Cell Death Discov; Loss of PML nuclear bodies in familial amyotrophic lateral sclerosis-frontotemporal dementia; Authors - Francesco Antoniani