SUMO1 Monoclonal antibody, PBS Only

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  WB analysis using 67559-1-Ig (same clone as 67559-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67559-1-Ig (SUMO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67559-1-Ig (same clone as 67559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67559-1-Ig (SUMO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 67559-1-Ig (same clone as 67559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67559-1-Ig (SUMO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

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  IHC staining of rat small intestine using 67559-1-Ig (same clone as 67559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 67559-1-Ig (SUMO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67559-1-Ig (same clone as 67559-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SUMO1 antibody (67559-1-Ig, Clone: 3B5B5 ) at dilution of 1:1600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 67559-1-Ig (same clone as 67559-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SUMO1 antibody (67559-1-Ig, Clone: 3B5B5 ) at dilution of 1:3200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

SUMO1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67559-1-PBS

Clone No.3B5B5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Small ubiquitin related modifier 1, Sentrin, OK/SW-cl.43, GMP1, GAP-modifying protein 1

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Product Information

67559-1-PBS targets SUMO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SUMO1 fusion protein Ag29402
• Full Name: SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 15-100 kDa
• GenBank Accession Number: BC006462
• Gene Symbol: SUMO1
• Gene ID (NCBI): 7341
• RRID: AB_2882773
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P63165
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.This antibody can detect endogenous levels of SUMOylated proteins (e.g. SUMO-1-RanGAP at 80-90 kD).