SULT2B1 Polyclonal antibody

SULT2B1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29185-1-AP

Synonyms

Alcohol sulfotransferase, HSST2, ST2B1, Sulfotransferase 2B1, SULT2B1

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Validation Data Gallery

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  WB analysis using 29185-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29185-1-AP (SULT2B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 29185-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 29185-1-AP (SULT2B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, mouse skin tissue, LNCaP cells, MCF-7 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29185-1-AP targets SULT2B1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SULT2B1 fusion protein Ag30209
• Full Name: sulfotransferase family, cytosolic, 2B, member 1
• Calculated Molecular Weight: 365 aa, 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC034694
• Gene Symbol: SULT2B1
• Gene ID (NCBI): 6820
• RRID: AB_2918245
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SULT2B1 is a member of hydroxysteroid sulfotransferase (SULT) family that catalyzes sulfonation of hormones and other molecules. SULT2B1 encodes two isoforms, SULT2B1a and SULT2B1b, that differ only at their amino termini but have distinct biochemical properties as well as tissue distribution. The human fetal brain appears to only express the SULT2B1a isoform, while SULT2B1b is the predominant hydroxysteroid sulfotransferase expressed in human skin. Northern blot analysis revealed SULT2B1a has a limited tissue distribution in liver, adrenal and stomach, whereas SULT2B1b can be detected in a variety of hormone-responsive tissues including placenta, ovary, uterus, and prostate.