SULT1A1 Polyclonal antibody

SULT1A1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 10911-2-AP

Synonyms

Phenol-sulfating phenol sulfotransferase 1, P PST 1, P PST, OK/SW-cl.88, HAST1/HAST2

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Validation Data Gallery

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  WB analysis of mouse liver using 10911-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10911-2-AP (SULT1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 10911-2-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10911-2-AP (SULT1A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 10911-2-AP

  IP result of anti-SULT1A1 (IP:10911-2-AP, 3ug; Detection:10911-2-AP 1:1000) with mouse liver tissue lysate 12500ug.

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  IHC staining of human ovary cancer using 10911-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10911-2-AP (SULT1A1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10911-2-AP

  Immunofluorescent analysis of HepG2 cells, using SULT1A1 antibody 10911-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 3 publications below

Product Information

10911-2-AP targets SULT1A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SULT1A1 fusion protein Ag1344
• Full Name: sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000923
• Gene Symbol: SULT1A1
• Gene ID (NCBI): 6817
• RRID: AB_2197593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SULT1A1, also named as ST1A1, P-PST 1, ST1A3, Ts-PST, P-PST 1, STP and STP1, belongs to the sulfotransferase 1 family. It catalyzes the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. SULT1A1 is also responsible for the sulfation and activation of minoxidil. It mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk. SULT1A1 is one of two phenol sulfotransferases with thermostable enzyme activity.

Publications

Species	Application	Title
human	WB	Oxid Med Cell Longev; Correlation of Reactive Oxygen Species Levels with Resveratrol Sensitivities of Anaplastic Thyroid Cancer Cells.; Authors - Xu Zheng
human,rat	WB,IHC	Biochem Pharmacol; Identification of metabolic pattern and bioactive form of resveratrol in human medulloblastoma cells.; Authors - Shu Xiao-Hong XH
human	WB	FEBS J; Distinct sulfonation activities in resveratrol-sensitive and resveratrol-insensitive human glioblastoma cells.; Authors - Sun Zheng Z
mouse	WB	Drug Metab Dispos; Sulfotransferase 4A1 (SULT4A1) increases its expression in mouse neurons as they mature.; Authors - Takuyu Hashiguchi
human	WB,IHC	PLoS One; Metabolic patterns and biotransformation activities of resveratrol in human glioblastoma cells: relevance with therapeutic efficacies.; Authors - Shu Xiao-Hong XH
human	IHC,WB	J Cancer; Increased Reactive Oxygen Species and Distinct Oxidative Damage in Resveratrol-suppressed Glioblastoma Cells.; Authors - Bin Jia