SUGT1 Polyclonal antibody

SUGT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11675-1-AP

Synonyms

Sgt1, Protein SGT1 homolog, Protein 40-6-3, Protein 40 6 3

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Validation Data Gallery

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  WB analysis of Jurkat using 11675-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11675-1-AP (SUGT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11675-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11675-1-AP (SUGT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11675-1-AP

  IP result of anti-SUGT1 (IP:11675-1-AP, 3ug; Detection:11675-1-AP 1:500) with HEK-293 cells lysate 3200ug.

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  IHC staining of human pancreas cancer using 11675-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11675-1-AP (SUGT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of A431 using 11675-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 11675-1-AP (SUGT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

11675-1-AP targets SUGT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUGT1 fusion protein Ag2275
• Full Name: SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 38 kDa and 41 kDa
• GenBank Accession Number: BC000911
• Gene Symbol: SUGT1
• Gene ID (NCBI): 10910
• RRID: AB_2197436
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Front Immunol; HSF1 Protects Sepsis-Induced Acute Lung Injury by Inhibiting NLRP3 Inflammasome Activation.; Authors - Xueyan Shi
human	IHC	Oncogenesis; The oncogenic role of the cochaperone Sgt1.; Authors - H Ogi
human	WB	PLoS One; Calcyclin Binding Protein/Siah-1 Interacting Protein Is a Hsp90 Binding Chaperone.; Authors - Agnieszka Góral
human	IHC	Int J Oncol; Overexpression of SUGT1 in human colorectal cancer and its clinicopathological significance.; Authors - Iwatsuki Masaaki M
human	WB	JCI Insight; Echinatin effectively protects against NLRP3 inflammasome-driven diseases by targeting HSP90.; Authors - Guang Xu