SUCLG2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SUCLG2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14240-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 14240-1-AP (same clone as 14240-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14240-1-AP (SUCLG2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14240-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 14240-1-AP (same clone as 14240-1-PBS)

  IP result of anti-SUCLG2 (IP:14240-1-AP, 4ug; Detection:14240-1-AP 1:600) with mouse kidney tissue lysate 4800ug. This data was developed using the same antibody clone with 14240-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 14240-1-AP (same clone as 14240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14240-1-AP (SUCLG2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14240-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 14240-1-AP (same clone as 14240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14240-1-AP (SUCLG2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14240-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14240-1-AP (same clone as 14240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 14240-1-AP (SUCLG2 Antibody) at dilution of 1:25. This data was developed using the same antibody clone with 14240-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14240-1-AP (same clone as 14240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 14240-1-AP (SUCLG2 Antibody) at dilution of 1:25. This data was developed using the same antibody clone with 14240-1-PBS in a different storage buffer formulation.

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Product Information

14240-1-PBS targets SUCLG2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUCLG2 fusion protein Ag5495
• Full Name: succinate-CoA ligase, GDP-forming, beta subunit
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC047024
• Gene Symbol: SUCLG2
• Gene ID (NCBI): 8801
• RRID: AB_2197187
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SUCLG2, encodes a GTP-specific beta subunit of succinyl-CoA synthetase. Succinyl-CoA synthetase catalyzes the reversible reaction involving the formation of succinyl-CoA and succinate. The deficiency of SUCLG2 represents an encephalomyopathic form of mtDNA depletion syndromes (PMID:30470562). Mutated SUCLG1 causes mislocalization of SUCLG2 protein, morphological alterations of mitochondria and an early-onset severe neurometabolic disorder. SUCLG2 is mainly expressed in liver, kidney, heart (PMID: 9765291).