SUCLA2 Monoclonal antibody

SUCLA2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C7E4

Cat no : 68549-1-Ig

Synonyms

A BETA, SCS betaA, SUCLA2

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Validation Data Gallery

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  WB analysis using 68549-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68549-1-Ig (SUCLA2 antibody) at dilution of 1:9900 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68549-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68549-1-Ig (SUCLA2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IF Staining of Caco-2 using 68549-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SUCLA2 antibody (68549-1-Ig, Clone: 2C7E4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

• Positive WB detected in: HepG2 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, mouse brain tissue
• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68549-1-Ig targets SUCLA2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SUCLA2 fusion protein Ag17967
• Full Name: succinate-CoA ligase, ADP-forming, beta subunit
• Calculated Molecular Weight: 463 aa, 50 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC027587
• Gene Symbol: SUCLA2
• Gene ID (NCBI): 8803
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUCLA2, β subunit of succinate-CoA ligase, contributes to energy production in mitochondria through the succinate-CoA ligase enzyme. Succinate-CoA ligase catalyses the reversible conversion of succinyl-CoA and ADP or GDP to succinate and ATP or GTP. SUCLA2 mutations cause global protein succinylation and mitochondrial DNA depletion (PMID: 33230181, PMID: 20301762). Mutations in SUCLA2 are also associated with SUCLA2-related mitochondrial DNA depletion syndrome, leigh syndrome, down syndrome (PMID: 23010432).