STYXL1 Polyclonal antibody

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  WB analysis using 16433-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16433-1-AP (STYXL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16433-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16433-1-AP (STYXL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 16433-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using STYXL1 antibody (16433-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

STYXL1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16433-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

MKSTYX, MK STYX, Map kinase phosphatase-like protein MK-STYX, Inactive dual specificity protein phosphatase MK-STYX, DUSP24

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Tested Applications

• Positive WB detected in: NCI-H1299 cells, PC-12 cells, mouse testis tissue, rat testis tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

16433-1-AP targets STYXL1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STYXL1 fusion protein Ag9743
• Full Name: serine/threonine/tyrosine interacting-like 1
• Calculated Molecular Weight: 313 aa, 36 kDa
• Observed Molecular Weight: 25-36 kDa
• GenBank Accession Number: BC024035
• Gene Symbol: STYXL1
• Gene ID (NCBI): 51657
• RRID: AB_3085490
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y6J8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STYXL1, Serine/threonine/tyrosine-interacting-like protein 1, is an inner mitochondrial membrane protein that plays a role in induction of stimulus-dependent apoptosis via the intrinsic apoptotic pathway (PMID: 21262771). STYXL1 catalyzes inactive phosphatase (PMID: 20180778, PMID: 23163895). By binding to G3BP1, STYXL1 inhibits the formation of G3BP1-induced stress granules (PMID: 20180778, PMID: 23163895).

Publications

Species	Application	Title
	WB	Int J Biol Sci; LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis.; Authors - Yan Fang
mouse	WB	Int Immunopharmacol; Linderalactone mitigates diabetic cardiomyopathy in mice via suppressing the MAPK/ATF6 pathway; Authors - Xue Han