STX5 Polyclonal antibody

STX5 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 26711-1-AP

Synonyms

SED5, STX5, STX5A, syntaxin 5



Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26711-1-AP targets STX5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STX5 fusion protein Ag25098
Full Name syntaxin 5
Calculated Molecular Weight 32 kDa, 34 kDa, 42 kDa
Observed Molecular Weight 35 and 42 kDa
GenBank Accession NumberBC012137
Gene Symbol STX5
Gene ID (NCBI) 6811
RRIDAB_2880609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for STX5 antibody 26711-1-APDownload protocol
IHC protocol for STX5 antibody 26711-1-APDownload protocol
IF protocol for STX5 antibody 26711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome

Authors - Hana Nůsková

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Wei (Verified Customer) (06-28-2020)

Double band for whole heart tissues. The upper band is much dense than the lower band.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart