Syntaxin 3 Polyclonal antibody

Syntaxin 3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 15556-1-AP

Synonyms

STX3, STX3A, syntaxin 3

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 15556-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15556-1-AP (Syntaxin 3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 15556-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 15556-1-AP (Syntaxin 3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 15556-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 15556-1-AP (Syntaxin 3 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 15556-1-AP

  IP result of anti-Syntaxin 3 (IP:15556-1-AP, 4ug; Detection:15556-1-AP 1:1000) with SH-SY5Y cells lysate 3600ug.

• 

  IHC staining of human kidney using 15556-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15556-1-AP (Syntaxin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15556-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15556-1-AP (Syntaxin 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, human plasma tissue, Neuro-2a cells
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• IP: See 3 publications below

Product Information

15556-1-AP targets Syntaxin 3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Syntaxin 3 fusion protein Ag7925
• Full Name: syntaxin 3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC007405
• Gene Symbol: STX3
• Gene ID (NCBI): 6809
• RRID: AB_2198667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin-3 (also known as STX3) is a member of the syntaxin. Syntaxin-3 is a significant apical targeting protein in the epithelial membrane and in exocytosis process; it also acts as a vesicle transporter by cellular receptor in neutrophils, which is crucial for protein trafficking event (PMID: 25630102). It has been reported that syntaxin-3 has an important role in the growth of neurites, and serves as a direct target for omega-6 arachidonic acid (PMID: 1659826).

Publications

Species	Application	Title
mouse	WB, IF	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
human	WB,IP	Mol Ther; circMRPS35 promotes malignant progression and cisplatin resistance in hepatocellular carcinoma; Authors - Peng Li
mouse	IF	PLoS Genet; BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.; Authors - Ying Hsu
mouse	IHC	Hum Mol Genet; Photoreceptor cilia, in contrast to primary cilia, grant entry to a partially assembled BBSome.; Authors - Ying Hsu
mouse	IF	Front Mol Neurosci; Farnesylation of the Transducin G Protein Gamma Subunit Is a Prerequisite for Its Ciliary Targeting in Rod Photoreceptors.; Authors - Celine Brooks
human	WB	J Biol Chem; Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.; Authors - Wulin Aerbajinai

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