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STX17 Recombinant antibody, PBS Only

STX17 Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4G3

Cat no : 81899-1-PBS

Synonyms

FLJ20651, STX17, syntaxin 17

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using STX17 Recombinant antibody (81899-1-RR)

    WB analysis of HepG2 using 81899-1-RR (same clone as 81899-1-PBS)

    WB result of STX17 antibody (81899-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STX17 transfected HepG2 cells. This data was developed using the same antibody clone with 81899-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using STX17 Recombinant antibody (81899-1-RR)

    WB analysis using 81899-1-RR (same clone as 81899-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81899-1-RR (STX17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81899-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using STX17 Recombinant antibody (81899-1-RR)

    IF Staining of HepG2 using 81899-1-RR (same clone as 81899-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using STX17 antibody (81899-1-RR, Clone: 4G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81899-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81899-1-PBS targets STX17 in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STX17 fusion protein Ag12134
Full Name syntaxin 17
Calculated Molecular Weight 302 aa, 33 kDa
Observed Molecular Weight 33-35 kD
GenBank Accession NumberBC111009
Gene Symbol STX17
Gene ID (NCBI) 55014
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. Syntaxin 17 (STX17) is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. STX17 may also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi.