Syntaxin 10 Polyclonal antibody

Syntaxin 10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA, IF

Conjugate

Unconjugated

Publications

2

Cat no : 11036-1-AP

Synonyms

Syntaxin-10, Syn10, STX10

---

Validation Data Gallery

• 

  WB analysis of HeLa using 11036-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11036-1-AP (Syntaxin 10 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11036-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11036-1-AP (Syntaxin 10 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 11036-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11036-1-AP (Syntaxin 10 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 11036-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11036-1-AP (Syntaxin 10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11036-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11036-1-AP (Syntaxin 10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, BxPC-3 cells
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

11036-1-AP targets Syntaxin 10 in WB, IHC, ELISA, IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Syntaxin 10 fusion protein Ag1501
• Full Name: syntaxin 10
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC017237
• Gene Symbol: Syntaxin 10
• Gene ID (NCBI): 8677
• RRID: AB_2198210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin 10 (STX10), also named as SYN10, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes.

Publications

Species	Application	Title
human	IF	Eur J Immunol; SNARE protein expression and localization in human cytotoxic T lymphocytes.; Authors - Pattu Varsha V
human	WB	Front Oncol; Identification of a novel MYC target gene set signature for predicting the prognosis of osteosarcoma patients; Authors - Deliang Gong