STT3B Polyclonal antibody

• Featured Product
• KD/KO Validated

STT3B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 15323-1-AP

Synonyms

STT3, STT, Source of immunodominant MHC-associated peptides homolog, SIMP, EC:2.4.99.18

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Validation Data Gallery

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  WB analysis using 15323-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15323-1-AP (STT3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15323-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15323-1-AP (STT3B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15323-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15323-1-AP (STT3B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15323-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15323-1-AP (STT3B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15323-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15323-1-AP (STT3B Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 15323-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15323-1-AP (STT3B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 15323-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15323-1-AP (STT3B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 15323-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15323-1-AP (STT3B Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HepG2 using 15323-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15323-1-AP (STT3B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: rat brain tissue, human brain tissue, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 2 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Product Information

15323-1-AP targets STT3B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STT3B fusion protein Ag2039
• Full Name: STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC015880
• Gene Symbol: STT3B
• Gene ID (NCBI): 201595
• RRID: AB_2198046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STT3B is a component of the N-oligosaccharyl transferase (OST) enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3B seems to be involved in complex substrate specificity

Publications

Species	Application	Title
human	WB	Nat Chem Biol; Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.; Authors - Cecilia Lopez-Sambrooks
human	WB	J Cachexia Sarcopenia Muscle; Comparative proteomic analyses of Duchenne muscular dystrophy and Becker muscular dystrophy muscles: changes contributing to preserve muscle function in Becker muscular dystrophy patients.; Authors - Daniele Capitanio
human	WB,CoIP	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis; Authors - Madison T. Wright
human	WB	MBio; Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex.; Authors - David L Lin
human	WB	EBioMedicine; Targeting conserved N-glycosylation blocks SARS-CoV-2 variant infection in vitro.; Authors - Hsiang-Chi Huang
human	WB	Cell Chem Biol; Editing N-Glycan Site Occupancy with Small-Molecule Oligosaccharyltransferase Inhibitors.; Authors - Natalia Rinis ; KO Validated