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  • Featured Product
  • KD/KO Validated

STT3A Monoclonal antibody, PBS Only

STT3A Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E2B12

Cat no : 66581-1-PBS

Synonyms

ITM1, Integral membrane protein 1, EC:2.4.99.18, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A, B5

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using STT3A Monoclonal antibody (66581-1-Ig)

    WB analysis of THP-1 using 66581-1-Ig (same clone as 66581-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66581-1-Ig (STT3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66581-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STT3A Monoclonal antibody (66581-1-Ig)

    IHC staining of human breast cancer using 66581-1-Ig (same clone as 66581-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66581-1-Ig (STT3A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66581-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STT3A Monoclonal antibody (66581-1-Ig)

    IHC staining of human breast cancer using 66581-1-Ig (same clone as 66581-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66581-1-Ig (STT3A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66581-1-PBS in a different storage buffer formulation.

Product Information

66581-1-PBS targets STT3A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STT3A fusion protein Ag27072
Full Name STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
Calculated Molecular Weight 705 aa, 81 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC020965
Gene Symbol STT3A
Gene ID (NCBI) 3703
RRIDAB_2881941
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

STT3A, also named as Integral membrane protein 1, belongs to the STT3 family. STT3A is expressed at high levels in placenta, liver, muscle and pancreas, and at very low levels in brain, lung and kidney. STT3A is a catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A. There are two isoforms of STT3A with molecular weight of 81 and 70 kDa. 66581-1-Ig antibody detects a protein around 65-70 kDa in SDS-PAGE which is similar to papers published. (PMID: 32005703, 25135935)