STMN4 Polyclonal antibody, PBS Only

STMN4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12027-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of human brain using 12027-1-AP (same clone as 12027-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12027-1-AP (STMN4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12027-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 12027-1-AP (same clone as 12027-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12027-1-AP (STMN4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12027-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 12027-1-AP (same clone as 12027-1-PBS)

  IP result of anti-STMN4 (IP:12027-1-AP, 3ug; Detection:12027-1-AP 1:1000) with mouse brain tissue lysate 6000ug. This data was developed using the same antibody clone with 12027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 12027-1-AP (same clone as 12027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12027-1-AP (STMN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 12027-1-AP (same clone as 12027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12027-1-AP (STMN4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 12027-1-AP (same clone as 12027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12027-1-AP (STMN4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 12027-1-AP (same clone as 12027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12027-1-AP (STMN4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 12027-1-AP (same clone as 12027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12027-1-AP (STMN4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12027-1-PBS in a different storage buffer formulation.

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Product Information

12027-1-PBS targets STMN4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STMN4 fusion protein Ag2657
• Full Name: stathmin-like 4
• Calculated Molecular Weight: 216 aa, 25 kDa
• Observed Molecular Weight: 22-30 kDa
• GenBank Accession Number: BC011520
• Gene Symbol: STMN4
• Gene ID (NCBI): 81551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RB3 (Stathmin-4), encoded by STMN4 gene, is a neuron-specific homologue of the SCG10/stathmin family proteins, possessing a unique N-terminal membrane-associated domain and the stathmin-like domain at the C terminus, which promotes microtubule (MT) catastrophe and/or tubulin sequestering. Stathmin is a ubiquitous cytosolic phosphoprotein, preferentially expressed in the nervous system, and described as a relay integrating diverse intracellular signaling pathways. RB3 is consistently up-regulated in differentiated neuroblastoma cells, and stable expression of transfected RB3 could induce differentiation, suggesting a role of RB3 during neuroblastoma differentiation. Moreover, increased RB3 is a unique response protein in the stathmin-related proteins following optical nerve axotomy and RB3 may be a critical target for neurodegenerative diseases. STMN4 has 4 isoforms with MW 25, 23, 22 and 19 kDa (refer to UniProt). Another 30 kDa isoform can be found by NCBI Protein Blast. (https://www.ncbi.nlm.nih.gov/protein/XP_005273709)