STMN2 Recombinant antibody, PBS Only

STMN2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230110B12

Cat no : 82925-2-PBS

Synonyms

SGC 10, SCGN10, SCGN 10, SCG10, SCG 10

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 82925-2-RR (same clone as 82925-2-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 82925-2-RR (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82925-2-PBS in a different storage buffer formulation.

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  IP experiment of SH-SY5Y using 82925-2-RR (same clone as 82925-2-PBS)

  IP result of anti-STMN2 (IP:82925-2-RR, 4ug; Detection:82925-2-RR 1:1500) with SH-SY5Y cells lysate 1240 ug. This data was developed using the same antibody clone with 82925-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82925-2-RR (same clone as 82925-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82925-2-RR (STMN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82925-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82925-2-RR (same clone as 82925-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82925-2-RR (STMN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82925-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 82925-2-RR (same clone as 82925-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STMN2 antibody (82925-2-RR, Clone: 230110B12 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82925-2-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 82925-2-RR (same clone as 82925-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using STMN2 antibody (82925-2-RR, Clone: 230110B12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 82925-2-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 82925-2-RR (same clone as 82925-2-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0 ug STMN2 Recombinant antibody (82925-2-RR, Clone:230110B12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82925-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82925-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82925-2-RR against Human STMN2 were performed. The affinity constant is 1.48 nM.

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Product Information

82925-2-PBS targets STMN2 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: STMN2 fusion protein Ag0929
• Full Name: stathmin-like 2
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 18-21 kDa
• GenBank Accession Number: BC006302
• Gene Symbol: STMN2
• Gene ID (NCBI): 11075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)