STK39 Recombinant antibody
STK39 Recombinant Antibody for IF, FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
230382G12
Cat no : 83081-3-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HepG2 cells |
Positive FC detected in | Daudi cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:125-1:500 |
Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
83081-3-RR targets STK39 in IF, FC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | STK39 fusion protein Ag34503 |
Full Name | serine threonine kinase 39 (STE20/SPS1 homolog, yeast) |
Calculated Molecular Weight | 59 kDa |
GenBank Accession Number | BC166614 |
Gene Symbol | STK39 |
Gene ID (NCBI) | 27347 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STK39 (Serine/Threonine Kinase 39) is a proline- and alanine-rich Ste20-related kinase (also named as SPAK), which is composed of a short N-terminal proline and alanine repeats (PAPA box), a kinase catalytic domain and a C-terminal regulatory domain (PMID: 16382158). In mammalians, STK39 plays an important role in ion homeostasis by regulating the cation chloride cotransporters' activities, which is crucial for the modulation of renal salt transport and blood pressure (PMID: 33500714). Notably, STK39 is also implicated in the regulation of tumor cell proliferation, migration, and invasion in multiple cancers, including osteosarcoma and cervical cancer (PMID: 34335956).
Protocols
Product Specific Protocols | |
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IF protocol for STK39 antibody 83081-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |