STK11/LKB1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

STK11/LKB1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29323-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 29323-1-AP (same clone as 29323-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29323-1-AP (STK11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29323-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 29323-1-AP (same clone as 29323-1-PBS)

  WB result of STK11/LKB1 antibody (29323-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STK11/LKB1 transfected MCF-7 cells. This data was developed using the same antibody clone with 29323-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 29323-1-AP (same clone as 29323-1-PBS)

  WB result of STK11/LKB1 antibody (29323-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STK11/LKB1 transfected A431 cells. This data was developed using the same antibody clone with 29323-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 29323-1-AP (same clone as 29323-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29323-1-AP (STK11/LKB1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29323-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 29323-1-AP (same clone as 29323-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29323-1-AP (STK11/LKB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29323-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 29323-1-AP (same clone as 29323-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29323-1-AP (STK11/LKB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29323-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 29323-1-AP (same clone as 29323-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29323-1-AP (STK11/LKB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29323-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 29323-1-AP (same clone as 29323-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 29323-1-AP (STK11/LKB1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29323-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 29323-1-AP (same clone as 29323-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 29323-1-AP (STK11/LKB1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29323-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 29323-1-AP (same clone as 29323-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29323-1-AP (STK11/LKB1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29323-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 29323-1-AP (same clone as 29323-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29323-1-AP (STK11/LKB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29323-1-PBS in a different storage buffer formulation.

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Product Information

29323-1-PBS targets STK11/LKB1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STK11/LKB1 fusion protein Ag30903
• Full Name: serine/threonine kinase 11
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC007981
• Gene Symbol: LKB1
• Gene ID (NCBI): 6794
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers. STK11 has 2 isoforms with MW of 49 kDa and 45 kDa, and can be detected as 50-54 kDa after posttranslational modification.