STK10 Polyclonal antibody, PBS Only

STK10 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25471-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of SKOV-3 using 25471-1-AP (same clone as 25471-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 25471-1-AP (same clone as 25471-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 25471-1-AP (same clone as 25471-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 25471-1-AP (same clone as 25471-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 25471-1-AP (same clone as 25471-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 25471-1-AP (same clone as 25471-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

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Product Information

25471-1-PBS targets STK10 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STK10 fusion protein Ag22169
• Full Name: serine/threonine kinase 10
• Calculated Molecular Weight: 968 aa, 112 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC070077
• Gene Symbol: STK10
• Gene ID (NCBI): 6793
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Lymphocyte-oriented kinase deficiency encoded by the serine/threonine kinase 10 (STK10) gene correlates with the intracellular adhesion molecule 1 (ICAM-1)/lymphocyte function associated antigen 1 (LFA-1) complex in aspirin hypersensitivity(PMID:21905501). STK 10 is also named as LOK and expressed as a 130-kDa protein, which was detected predominantly in lymphoid organs such as spleen, thymus, and bone marrow, in contrast to other mammalian members of the STE20 family(PMID:9278426).