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STIM1 Recombinant antibody, PBS Only

STIM1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B13

Cat no : 81156-1-PBS

Synonyms

STRMK, stim-1, STIM 1, IMD10, GOK

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STIM1 Recombinant antibody (81156-1-RR)

    WB analysis using 81156-1-RR (same clone as 81156-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81156-1-RR (STIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81156-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using STIM1 Recombinant antibody (81156-1-RR)

    WB analysis using 81156-1-RR (same clone as 81156-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81156-1-RR (STIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using STIM1 Recombinant antibody (81156-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 81156-1-RR (same clone as 81156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 81156-1-RR (STIM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81156-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using STIM1 Recombinant antibody (81156-1-RR)

    IF Staining of HEK-293 using 81156-1-RR (same clone as 81156-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using STIM1 antibody (81156-1-RR, Clone: 1B13 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81156-1-PBS in a different storage buffer formulation.

Product Information

81156-1-PBS targets STIM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STIM1 fusion protein Ag2140
Full Name stromal interaction molecule 1
Calculated Molecular Weight 685 aa, 77 kDa
Observed Molecular Weight77 kDa
GenBank Accession NumberBC021300
Gene Symbol STIM1
Gene ID (NCBI) 6786
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.