STEAP4 Monoclonal antibody

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  WB analysis using 68465-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68465-1-Ig (STEAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 68465-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68465-1-Ig (STEAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

STEAP4 Monoclonal Antibody for WB, ELISA

Cat No. 68465-1-Ig

Clone No.2A12G6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

STEAP family member 4, STAMP2, Six-transmembrane epithelial antigen of prostate 4, Metalloreductase STEAP4, EC:1.16.1.-

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Tested Applications

• Positive WB detected in: A549 cells, rat liver tissue, HaCaT cells, HeLa cells, MDA-MB-468 cells, SK-BR-3 cells, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68465-1-Ig targets STEAP4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: STEAP4 fusion protein Ag30137
• Full Name: STEAP family member 4
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC020600
• Gene Symbol: STEAP4
• Gene ID (NCBI): 79689
• RRID: AB_3085176
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q687X5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STEAP4 (also called STAMP2), is a member of the STEAP family. Structurally, It is a 459 amino acid protein characterized by a molecular topology of six transmembrane domains. The cytoplasmic N-terminal domain of STEAP4 shows structural similarity with bacterial and archaeal FNO oxidoreductase and STEAP4 exhibits a strong iron reductase activity. Studies in mice and human suggest that STEAP4 maybe involved in adipocyte development and metabolism, and may contribute to the normal biology of the prostate cell, as well as prostate cancer progression.